PAAIS Research center

2. Pollen Research Center established with he collaboration of Allergy and Asthma Center Islamabad in 2015

We collect and purify pollen with following two methods with stringent methods of collection and purification and microscopical studies

  • Plants are collected according to season and placed on the large hanging aluminium trays where the sunlight falling from glass windows. When dry the flowers and catkins are put on the collecting trays and passed through different grades of sieves with shaker.
  • We have developed special turff of steel where branches with flowers of allergenic plants are put and the branch lower part is dipped in fresh water and upper part drooping out so that pollens fall on the filter paper around the turf. These are covered with plexiglass hood and exposed to lights led light (460 and 660 nm wave lengths). The pollen is collected on the white filter paper around the turf

CANNABIS Pollens in FALL and PAPER MULBERRY POLLENS in SPRING are causing severe allergic diseases in Islamabad. The Pollen count ins sprint rise to more than 35000 pollens per day or 1500 pollens per cubic meter per hour.
PAAIS with the collaboration of Allergy and Asthma Centre developed POLLEN RESEARCH CENTER. Where the branches are collected early morning with special devices. Pollens are collected by drying and developed special turf of steel having water Special turf for collection of Pollens. Branches with flowers or catkins are placed in turf of water and the upper parts of the branches are hanging or drooping out. These are exposed for some days to special lights which are helpful for dehiscence or release of pollens. These pollens fall on the glazed paper put below the turf.

3. House dust mite Research Center: -

House dust mite research centre was established in 2017 but in spite of efforts we could not culture the house dust mites. The Dr.Shahid Abbas had training in 2019 with Prof Anchalee and Prof Natt in Mahidol University Hospital, Department of Entomology, Bangkok and then imported special Humidifying Incubator and equipment and started culturing the House Dust Mite. Now we have some successes and we also have opened for training of doctors in house dust mite allergy


After identification house dust mites are cultured in humidifying incubator and observed frequently. Finally, the house dust mite colonies are separated and frozen for further research on house dust mite allergies




  • World Allergy Organization Study on Aerobiology for Creating First Pollen and Mold Calendar With Clinical Significance in Islamabad, Pakistan; A Project of World Allergy Organization and Pakistan Allergy, Asthma & Clinical Immunology Centre of Islamabad WAO Journal(BIoMediCenter)
  • Role of exons of intercellular adhesive molecules (ICAM-1) in patients suffering from Allergic Rhinitis in Pakistan
  • The effect of residential urban greenness on allergic respiratory diseases in youth: A narrative review
    La Grutta S et al 2020;13(1):100009
  • International expert consensus on the management of allergic rhinitis (AR) aggravated by air pollutants Naclerio R et al World Allergy Organ J
  • International expert consensus on the management of allergic rhinitis (AR) aggravated by air pollutants
    Naclerio R et al
    World Allergy Organ J 2020;13(3):100106
  • Air pollution and indoor settings
    Rosario Filho N et al
    World Allergy Organ J 2021;14(1):100499
  • Allergic rhinitis aggravated by air pollutants in Latin America: A systematic review
    Rosario Filho NA et al
    World Allergy Organ J 2021;14(8):100574
  • Decreasing ten-year (2008–2018) trends of the prevalence of childhood asthma and air pollution in Southern Taiwan
    Chen WY et al
    World Allergy Organ J 2021;14(5):100538
  • Immunopathological features of air pollution and its impact on inflammatory airway diseases (IAD)
    Rouadi PW et al
    World Allergy Organ J 2020;13(10):100467
  • Ambient particulate matter associates with asthma in high altitude region: A population-based study
    Xing ZZ et al
    World Allergy Organ J 2023;16(5):100774

Research trainings:

  1. Internship
  2. Research Project


Research student registration form: Download here